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- Asahipak ES系列的分离条件
- 点击次数:577 更新时间:2023-09-26
- 可以作为Asahipak ES系列流动相的缓冲液盐浓度为20~600 mM。不要使用纯水,因为纯水会使色谱柱劣化。具体请参考下表。可以使用NaCl, KCl, Na2SO4和K2SO4 等的水溶液也可以和缓冲溶液配合使用。
pH range Buffer 3.8 - 4.3 Sodium formate 4.3 Sodium succinate 4.8 - 5.2 Sodium acetate 5.0 - 6.0 Sodium malonate 5.5 - 6.7 MES 6.7 - 7.6 Sodium phosphate 7.6 - 8.2 HEPES 8.2 - 8.7 BICINE pH range Buffer 4.8 - 5.0 N-Methylpiperazine HCl 5.0 - 6.0 Piperazine HCl 5.8 - 6.4 Bis Tris HCl 6.4 - 7.3 Bis Tris Propane HCl 7.3 - 7.7 Triethanolamine HCl 7.5 - 8.0 Tris HCl 8.0 - 8.5 N-Methyldiethanolamine HCl 8.4 - 8.8 Diethanolamine HCl 8.5 - 9.0 1,3-Diaminopropane HCl 9.0 - 9.5 Ethanolamine HCl - MES: 2-(N-morpholino)ethanesulfonic acid
HEPES: 4-(2-hydroxymethyl)-1-piperazine ethanesulfonic acid
BICINE: N,N-bis(2-hydroxyethyl)glycine
Tris: tris(hydroxymethyl)aminomethane
Bis Tris : Bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane
Bis Tris propane: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
- MES: 2-(N-morpholino)ethanesulfonic acid